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Svevo x Zavitan Population
The Mapping population consisting of 150 Recombinant Inbreed Lines (RILs) developed by Single Seed Decent (SSD) from a cross between elite durum wheat (cv. Svevo) and highly drought tolerant wild emmer (acc. Zavitan). This mapping population is based on high yielding durum cultivar Svevo, with good baking quality, well adapted to the Mediterranean environment. The wild emmer accession, Zavitan, was collected in Israel and tested for drought resistance in the field and laboratory, confirming high tolerance to drought; improved water-used efficiency and yield. The mapping population have been genotyped using the high throughput 90K-SNP array chip (Illumina) to produce a high density genetic map which serve as platform for mapping of QTLs associated with drought and heat resistance.
Spikelet and grains of the two parental lines of the RILs mapping populations: Triticum turgudum ssp. durum cultivar Svevo and Triticum turgidum ssp. dicoccoides accession Zavitan.
Genotyping
The wheat 90K iSelect SNP genotyping assay was used for genotyping of the RILs, detecting segregation for 16,387 polymorphic markers. The genetic map was constructed based on the genotypic data of 140 RILs and included a total of 14,088 markers grouped into 2,296 genetic loci (unresolvable by recombination) in 14 linkage groups, corresponding to the 14 chromosomes of tetraploid wheat. The map was 2,110 cM long with an average distance of 0.92 cM between adjacent markers. This ultra-dense SNP-based genetic map with a high level of synteny to barley provides a useful framework for genetic analyses of important traits, positional cloning and markerassisted selection, as well as for comparative genomics and genome organization studies in wheat and other cereals.
To read more see Avni et al. (2014) Mol Breeding, 34:1549-1562.
Comparison between Zavitan (on the left) and svevo.
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